All lanes : Anti-ZEB1 antibody [BLR102H] (ab276129) at 1/1000 dilution Lane 1 : C2C12 whole cell lysate Lane 2 : 70Z/3 whole cell lysate Lane 3 : BW5147.3 whole cell lysate Lane 4 : CTLL-2 whole cell lysate Lane 5 : EL4 whole cell lysate Lane 6 : RenCa whole cell lysate Lane 7 : NIH/3T3 whole cell lysate Lane 8 : TCMK-1 whole cell lysate Lane 9 : A20 whole cell lysate This product is for research use only and is not approved for use in humans or in clinical diagnosis. These antibodies target ZEB1 in Human, Mouse and Rat samples. 100% Guaranteed. . Monoclonal Rabbit Anti Zeb1, supplied by Cell Signaling Technology Inc, used in various techniques. Available in 2 sizes. Anti-ZEB1 antibody . ZEB1 and ZEB2 contain two separate zinc-finger domains and a homeodomain (1). Log in / Register . Monoclonal antibody is produced by immunizing animals with recombinant protein corresponding to the central region of human ZEB1 protein. This antibody recognizes Human antigen. Beta Catenin in Cell Adhesion and T-cell Signaling Beta Catenin is a cytosolic, 88 kDa intracellular protein that tightly associates with cell . Anti Zeb1 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. 33 Soluble proteins were subjected to immunoprecipitation with anti-ZEB1 antibody (Cell Signaling Technology, Danvers, MA, USA). ZERO BIAS - scores, article reviews, protocol conditions and more. . One of the targets suppressed by ZEB proteins is E-cadherin. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated ZEB1 (E2G6Y) XP Rabbit mAb #70512. ZEB1 (zinc-finger E-box-binding homeobox 1), also known as TCF8 (transcription factor 8), is a delta-EF1/ZFH-1 C2H2-type zinc finger that bears seven C2H2-type zinc fingers and one homeobox DNA-binding domain. Home > Search Results > Cell Signaling Technology Inc > tcf8 zeb1 antibodies. Background ZEB family proteins are zinc-finger and homeobox domain containing transcription factors. This target gene encodes the protein 'zinc finger E-box binding homeobox 1' in humans and may also be known as zeb, AREB6, TCF8, BZP, DELTAEF1, and delta-crystallin enhancer binding factor 1. Highly specific and rigorously validated in-house, ZEB1 (D80D3) Rabbit Monoclonal Antibody (CST #3396) is ready to ship. Chromatin immunoprecipitations were performed with cross-linked chromatin from HCT 116 cells and either -Catenin (D10A8) XP Rabbit mAb or Normal Rabbit IgG #2729 using SimpleChIP Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. There are two members in mammals, ZEB1 (-EF1, TCF8, AREB6) and ZEB2 (SIP1). ZERO BIAS - scores, article reviews, protocol conditions and more EMT induced by loss of LKB1 promotes migration and invasion of liver cancer cells through ZEB1-induced YAP signaling Qiu B et al Oncol Lett 2018;16(5):6465-6471 Western Blot analysis of ZEB1 in HeLa cell lysate with ZEB1 Polyclonal Antibody (Product # PA5-20979) at 1 g/mL. 8776162355. headquarters: USA. Validated for Western Blotting, Immunoprecipitation. ZEB1 is a transcriptional repressor of interleukin-2 (IL-2). #9782. The ZEB1 (D80D3) Rabbit mAb Antibody from Cell Signaling Technology is a Rabbit Monoclonal antibody to Zeb1. elife (2016) ncbi Product Usage Information Storage Please check that this product meets your needs before purchasing. Antibody information for antibodies HPA027524, CAB058686 and CAB079943 used in analysis of ENSG00000148516 / ZEB1 (AREB6, BZP, FECD6, NIL-2-A, PPCD3, TCF8, ZEB, Zfhep, Zfhx1a) . Bioz Stars score: 96/100, based on 2 PubMed citations. human ZEB1 antibody; mouse Zeb1 antibody; rat Zeb1 antibody; browse more . Epigenetics and Nuclear Signaling Transcription Domain Families Zinc Finger. ZERO BIAS - scores, article reviews, protocol conditions and more Primary antibody was incubated over night at 4 C (active -catenin 1:1000 in 5% BSA/TBST (non-phospho/active -catenin, Ser33/37/Thr41, rabbit mAb; Cell Signaling, Danvers, MA, USA)). Validated for WB, IP, IHC, IHC, IF, F. Available in 2 sizes. ZERO BIAS - scores, article reviews, protocol conditions and more Rabbit ZEB1 Rabbit pAb (A16981), validaed in WB,IHC,IF and tested in Human,, Mouse,, Rat. Immunoprecipitation of ZEB1 protein from Jurkat cell extracts. In addition, YTHDF2 is essential for the posttranscriptional regulation of ZEB1 by piRNA-17560/FTO signaling. Replacement to Abcam, Santa Cruz, Sigma and CST antibody. Bioz Stars score: 96/100, based on 1 PubMed citations. Senescent neutrophils secret exosomal piR-17560 in a STAT3-dependent manner. Background ZEB family proteins are zinc-finger and homeobox domain containing transcription factors. The enriched DNA was quantified by real . . ( A ) miR-205-NPs treatment suppresses the migratory ability of C4-2 and PC-3 cells in presence/absence of the drug through Boyden chamber study. Bioz Stars score: 96/100, based on 1 PubMed citations. In mice, Zeb1 induced increased cadherin and Integrin expression in the gastrocnemius muscle following an injection with an adenovirus vector expressing Nr4a1(Nr4a1 . Our ZEB1 monoclonal, polyclonal and recombinant monoclonal antibodies are developed in Mouse, Rabbit and Goat. ZEB1 antibodies are useful tools for studying certain degenerative ocular diseases. [supplied by OMIM] Limitations Bioz Stars score: 95/100, based on 1 PubMed citations. Cited in 20 publications. The ZEB1 (D80D3) Rabbit mAb Antibody has been validated for the following applications: Precipitation, Immunoprecipitation, and Western Blot. Cell Signaling Technology Inc anti zeb1 3396s antibodies Anti Zeb1 3396s Antibodies, supplied by Cell Signaling Technology Inc, used in various techniques. ZERO BIAS - scores, article reviews, protocol conditions and more. Highly specific and rigorously validated in-house, ZEB1 (E2G6Y) XP Rabbit Monoclonal Antibody (CST #70512) is ready to ship. 40 ug 293T whole cell lysate/extract B. Total cell lysates were prepared in NP-40 lysis buffer as described. Bioz Stars score: 86/100, based on 1 PubMed citations. All lanes : Anti-ZEB1 antibody (ab245283) at 0.1 g/ml Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell . Cited in 354 publications. We are dedicated to providing innovative research tools that are used to help define mechanisms underlying cell function and disease. Figure 6 miR-205-NPs inhibits EMT signaling and sensitizes Dtxl treatment in PrCa cells. . While ZEB proteins mainly function as transcriptional suppressors, they are able to activate transcription, dependent on DNA-context and cell type (1). Bioz Stars score: 96/100, based on 17 PubMed citations. In order to research induction of epithelial-to-mesenchymal transition by Polo-like kinase 1 and promotion of epithelial cell motility by activating CRAF/ERK signaling, ProSci ZEB1 antibody (ProSci Inc, 5825) was used in western blot on human samples at 1:250 (fig 5). B Cell Receptor Signaling Pathway; T Cell Receptor Signaling Pathway; Jak-Stat-IL-6 . Share by email . We are dedicated to providing innovative research tools that are used to help define mechanisms underlying cell function and disease. Epithelial-Mesenchymal Transition (EMT) Antibody Sampler Kit. Beta Catenin in Cell Adhesion and T-cell Signaling Beta Catenin is a cytosolic, 88 kDa intracellular protein that tightly . Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of ZEB1 protein by 2 ug of . Monoclonal Antibody for studying ZEB1/TCF8. ZEB1 is a zinc finger transcription factor that represses T-lymphocyte-specific IL2 gene expression by binding to a negative regulatory domain 100 nucleotides 5-prime of the IL2 transcription start site. Western blot analysis was performed using ZEB1 (E2G6Y) XP Rabbit mAb. Established in Beverly, MA in 1999, Cell Signaling Technology (CST) is a privately-owned company with over 400 employees worldwide. Subsequently, the samples were washed thoroughly using TBST and incubated for 1 h at room temperature with a secondary antibody goat anti-rabbit IRDye800CW (1 . Established in Beverly, MA in 1999, Cell Signaling Technology (CST) is a privately-owned company with over 400 employees worldwide. Anti-ZEB1 antibody [EPR17375] (ab203829) Research with confidence - consistent and reproducible results with every batch Long-term and scalable supply - powered by recombinant technology for fast production Success from the first experiment - confirmed specificity through extensive validation Ethical standards compliant - production is animal-free zeb1 antibody (Cell Signaling Technology Inc) 94. ZEB1 encodes a zinc finger transcription factor that represses T-lymphocyte-specific IL2 gene (MIM 147680) expression by binding to a negative regulatory domain 100 nucleotides 5-prime of the IL2 transcription start site (Williams et al., 1991 [PubMed 1840704]). Validations Orthogonal Applications ZEB1 Modification Unmodified View Product On Supplier's Website Request a Quote from Cell Signaling Technology Supplier provided information Immunogen Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to a region around residue Asp868 of human TCF8/ZEB1. Antibodies that detect ZEB1 can be used in several scientific applications, including Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation and ChIP. Immunoprecipitation: ZEB1 Antibody [NBP2-20942] - 293T whole cell lysate/extract A. Home > Search Results > Cell Signaling Technology Inc > anti zeb1 antibody. Anti-rabbit IgG, HRP-linked Antibody #7074 was used as the secondary antibody. Limitations. Cell Signaling Technology Inc polyclonal zeb1 antibody Polyclonal Zeb1 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Tcf8 Zeb1 Antibodies, supplied by Cell Signaling Technology Inc, used in various techniques. ABclonal provides trial size antibody samples for target detection. Monoclonal Antibody for studying ZEB1/TCF8. Since its inception, CST has become the world . Anti-Zeb1 Antibody Products from Cell Signaling Technology Anti-ZEB1 antibodies are offered by a number of suppliers. Moreover, Nr4a1 increased myoblast adhesion via directly binding to an upstream site of zinc finger E-box binding homeobox 1 (Zeb1), which is required for myogenesis in myoblasts. Limitations This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP Isotype Control #3900, and lane 3 is ZEB1 (E2G6Y) XP Rabbit mAb. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. This Cell Signaling Technology antibody is conjugated to Alexa Fluor 647 fluorescent dye and tested in-house for direct flow cytometric analysis in human cells. There are two members in mammals, ZEB1 (-EF1, TCF8, AREB6) and ZEB2 (SIP1). Monoclonal antibody is produced by immunizing animals with recombinant protein corresponding to the central region of human ZEB1 protein. Cell Signaling Technology Inc rabbit monoclonal anti zeb1 Rabbit Monoclonal Anti Zeb1, supplied by Cell Signaling Technology Inc, used in various techniques. Precipitates and cell lysates were analyzed by immunoblotting with anti-MUC1-C (LabVision, Fremont, CA, USA), anti-NF-B (Santa Cruz Biotechnology . ZERO BIAS - scores, article reviews, protocol conditions and more.
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