K lambda positive cells were also observed. Article Title: Utility of VS38c in the diagnostic and prognostic assessment of osteosarcoma and other bone tumours/tumour-like lesions. The antibody reacts strongly with human plasma cells (1). Results: All four cases exhibited similar morphology and a largely consistent immunohistochemical staining profile. IMMUNOHISTOCHEMISTRY AND CHROMOGENIC IN SITU (CISH) SPECIAL STAINS ACTH polyclonal Actin, Muscle Specific HHF35 Actin Smooth Muscle 1A4 Adenovirus M58+M73 AFP polyclonal . VS38c with high intensity either with immunohistochemistry or with FCM in BM samples [10, 12]. In this review, we try to summarize the current state of the art in BM immunohistochemistry for the diagnosis of hematologic disorders. W. WT1 (6F-H2) Wilms tumor. IMMUNOHISTOCHEMISTRY AND CHROMOGENIC IN SITU (CISH) SPECIAL STAINS ACTH polyclonal Actin, Muscle Specific HHF35 Actin Smooth Muscle 1A4 Adenovirus M58+M73 AFP polyclonal . IgM secreting plasma cell neoplasms are exceedingly rare, meanwhile CD20 is expressed in up to 20% of plasma cell neoplasms ( Am J Hematol 2010;85:853 ). F 7149 Edition/ Edition/ Ausgabe 01.11.02 Intended use For in vitro diagnostic use. 1-9. Pathologists at our institution use the panel with VS38 at their discretion for routine clinical care in cases from patients with a known history of daratumumab therapy or with dim CD38 . While present in this case, CD7 positivity is not typical of this disease. We found no evidence of plasmacytic differentiation by CD138, and VS38c immunoreactivity was distinctly rare (2 of 29 cases). mal and neoplastic plasma cells by immunohistochemistry. It is a sensitive marker for plasma cells. The focus of the sixth edition of the IHC Guidebook is to provide a comprehensive immunohistochemistry (IHC) resource for lab managers, lab technicians, learning pathologists, and students from around the world. Chromogenic in situ Hybridization . It is performed on thinly sliced formalin-fixed paraffin embedded (FFPE) tissue mounted on slides and interpreted using a microscope. Results Description Code M7077 Monoclonal Mouse Anti-Human Plasma Cell, Clone VS38c, Concentrated Antibody for Manual Use, Unconjugated, Culture supernatant, Immunohistochemistry, 1 mL. Actin HHF35 Human Muscle Actin. Limitations of VS38c labeling in the detection of plasma cell myeloma by flow cytometry - August 21, 2021 - Dominique s were subclassified according to the criteria of the WHO classification and evaluated by immunohistochemistry for expression of antigens associated with germinal center (GC) and non-GC stages of B-cell differentiation (bcl-6, CD10, MUM-1, VS38c, CD138, bcl-2, and CD44). In addition, VS38c staining of cultures of MG63 and Sa0S2 osteosarcoma cell cultures. ZERO BIAS - scores, article reviews, protocol conditions and more CD38 CD138 CD20 IgM VS38c Board review style answer #1 D. IgM is the best answer. Results F 7149 is intended for use in flow cytometry. Immunohistochemistry Stains. Since plasma cells show uniquely strong staining with VS38c among hema-topoietic cells, it is proposed by Mizuta et al. A. ACTH Human Adrenocorticotropic Hormone. Moreover, diagnostic algorithms and useful antibody panels are proposed for a rational and cost-effective approach. Mizuta and colleagues 7 evaluated VS38c in 15 patients with MM and showed that this antibody enabled detection of plasma cells in all cases, including in 3 patients who had received daratumumab. CD44 was detected in 6 tumors, all CD10-. The presence of a BCL-2/IgH gene rearrangement was investigated by polymerase chain reaction. . VS38c is a monoclonal antibody clone (mAb) that recognizes a luminal epitope of the endoplasmic reticulum (ER) resident cytoskeleton-linking membrane protein 63 (CLIMP-63, also known as cytoskeleton-associated protein 4, CKAP4, or p63) [ 10 ]. as an intracellular IMMUNOHISTOCHEMISTRY AND CHROMOGENIC IN SITU (CISH) ACTH polyclonal Actin, Muscle Specific HHF35 Actin Smooth Muscle 1A4 ADH5 DS Breast Cancer Adenovirus M58+M73 . (cisplatin and actinomycin D-treatment) was analysed. Whereas VS38c staining was strong in both normal plasma cells and MM cells, independent of daratumumab treatment, staining intensities for CD38 were lower in MM cells compared with normal plasma cells, and on both cell types CD38 expression was significantly reduced in daratumumab-treated patients. (cisplatin and actinomycin D-treatment) was analysed. Immunohistochemistry (IHC) is the most common application of immunostaining.It involves the process of selectively identifying antigens (proteins) in cells of a tissue section by exploiting the principle of antibodies binding specifically to antigens in biological tissues. VS38C VS38c WT1 6F-H2 CISH EBER CISH HPV Low Risk CISH HPV High Risk CISH KAPPA CISH LAMBDA Antibody Clone GROUP I (Microorganisms) VS38 VS38 Immunohistochemical expression This commercially available antibody is directed against a protein of the endoplasmic reticulum. Our flow cytometry laboratory began using the monoclonal antibody VS38 after an internal laboratory validation process. The VS38c antibody has been shown to identify the human p63 protein (2, 3). Staining for Vs38c (A) demonstrate the mainly perivascularly located clusters of plasma cells. VS38C Plasma Cell. Immunohistochemistry (IHC) uses antibodies to detect the location of proteins and other antigens in tissue sections. Immunohistochemical staining with VS38c was carried out on formalin-fixed specimens of osteosarcoma (pre/post-chemotherapy) and a wide range of benign and malignant bone lesions. All cases were characterized . VS38c and PE were obtained from Dako. The neoplastic cells expressed recently described plasma cell-associated antigens CD138 and VS38c.12,13 This immunophenotype is typical and similar to the previously described immunophenotypic findings in PBL. Interpretation of results must be made within the Methods: ImmunohistochemicalstainingwithVS38cwascarriedoutonformalin-xedspecimensofosteosarcoma (pre/post-chemotherapy)andawiderangeofbenignandmalignantbonelesions.Inaddition,VS38cstainingofcul- turesofMG63andSa0S2osteosarcomacellcultures. This antibody has been shown to work in applications such as: Immunohistochemistry. Definition / general A carbohydrate (not a protein) widely used for diagnosis of Hodgkin lymphoma Also known as LeuM1, Lewis X, 3-fucosyl-N-acetyl-lactosamine Mediates phagocytosis and chemotaxis Synthesis is directed by FUT4 ( OMIM #104230) in lymphoid cells and mature granulocytes, and by FUT9 ( OMIM #606865) in promyelocytes and monocytes Bioz Stars score: 99/100, based on 1 PubMed citations. 22 Briefly, following blockade of endogenous peroxidase, VS38C. VS38c staining of plasma cells is reported t o be constant and strong even in myeloma, we . Positive staining is seen in plasma cell, plasmablasts, lymphoplasmacytoid cells, and B-immunoblasts. Immunohistochemistry (IHC) is a technique that uses antibodies applied to tissues to detect targets of interest-usually a specific protein (antigen). Both marked with CD38, CD138, and VS38c, recognized markers for plasma cells; however, EMA and CD31 were present only in the classic form of plasma cell. Although less quantitative than assays such as . Troubleshooting. Clone VS38c Code No./ Code/ Code-Nr. CLIMP-63 is known to be expressed at high levels in normal and pathologic plasma. Immunohistochemical staining with VS38c was carried out on formalin-fixed specimens of osteosarcoma (pre/post-chemotherapy) and a wide range of benign and malignant bone lesions. It has been used in immunohistochemistry for a long time, 9 but only recently has it been used more widely in flow cytometry. The antibody-antigen interaction is visualized using either chromogenic detection with a colored enzyme substrate, or fluorescent detection with a fluorescent dye. ZERO BIAS - scores, article reviews, protocol conditions and more VS38C VS38c WT1 6F-H2 CHROMOGENIC ISH PROBES CISH EBER CISH HPV Low Risk (6 Subtypes) CISH HPV High Risk (18 subtypes) CISH KAPPA CISH LAMBDA Figure Lengend Snippet: Immunohistochemistry with monoclonal antibody VS38c showing ( a ) staining of cultured SAOS2 osteosarcoma cells and ( b ) SAOS2 cells treated with . Immunohistochemistry is a technique that uses antigen-antibody interactions to detect specific proteins in cells. The definitive evidence that molecular genetic analysis showed a clonal single electrophoretic band of SYT-SSX mutated chimera gene was conclusive for the pathological diagnosis. VS38c is a monoclonal antibody that recognizes the CLIMP-63 protein in the membrane of the endoplasmic reticulum. Tumor cells were stained with vimentin and the plasma cell markers, VS38c, CD138 . Bcl-2 was expressed by 70% of the tumors, but only 1 of 23 cases tested had a Bcl-2/JH rearrangement by polymerase chain reaction. In addition, VS38c staining of cultures of MG63 and Sa0S2 osteosarcoma cell cultures. Immunohistochemical staining with VS38c was carried out on formalin-fixed specimens of osteosarcoma (pre/post-chemotherapy) and a wide range of benign and malignant bone lesions. Results VS38c is a monoclonal antibody that recognizes the CLIMP-63 protein in the membrane of the endoplasmic reticulum. Which of the following immunohistochemical markers has the lowest sensitivity for plasma cell neoplasms? Immunohistochemistry revealed neither lymphoma markers nor clonal cytoplasmic nor cell surface immunoglobulins. To resolve this contradiction, we first checked whether the permeabilization of the Both tumor types showed immunoreactivity for various cytokeratins, epithelial membrane antigen (EMA), vimentin, neurofilaments, S100 protein, neuron specific enolase, Leu7, CD34, p53, MB2, MIC2, VS38c, laminin and fibronectin. Applied Immunohistochemistry & Molecular Morphology: June 2005 - Volume 13 - Issue 2 - p 124-131. doi: 10.1097/01.pai.0000135614.30196.fb . The patient was HHV-8-negative. CLIMP-63 is known to be expressed at high levels in normal and pathologic plasma cells in the bone marrow, thus VS38c antibody can be used to identify them. The resected specimen was positive for T cell markers (CD3 CD5), a B cell marker (CD20), B cell and plasma cell markers (CD79a, CD138), and a plasma cell marker (VS38c), as assessed by immunohistochemistry. This technique has several essential applications in lymphoma diagnosis, including identifying the cell lineage and phase of maturation, detecting specific genetic alterations, visualizing the degree of cell proliferation, and identifying therapeutic targets. Immunohistochemistry is positive for HHV8 (essential for diagnosis), plasma cell associated markers (CD138, VS38c, CD38 , EMA, IRF-4/MUM 1), CD30, CD45; variable for cytoplasmic Ig light chain and negative for pan B cel markers (CD19, CD20, CD79 and Pax-5). CISH EBER Epstein-Barr Virus (EBV) Recognizes an intracellular protein of 63 kDa identical with the rough endoplasmic . Vs38c Mab, supplied by Agilent technologies, used in various techniques. Furthermore, positive immunostains for MEF2, VS38c (plasma cell antigen), and bcl-2 were rather significant findings in the present case. The proliferation marker Ki67 was present in less . ( cisplatinandactinomycinD-treatment)wasanalysed. IHC takes its name from the roots "immuno", in reference to antibodies used in the procedure, and "histo", meaning tissue . Polymerase chain reaction amplification of immunoglobulin heavy-chain gene showed a monoclonal band that supports the B-cell nature of . Background VS38c is a monoclonal antibody that recognises a rough endoplasmic reticulum (rER) intracellular antigen termed cytoskeleton-linking membrane protein 63. rER is typically found in. (cisplatin and actinomycin D-treatment) was analysed. . 2SC Hereditary Leiomyomatosis and Renal Cell Carcinoma (HLRCC) Syndrome. VS38C VS38c WT1 6F-H2 CHROMOGENIC ISH PROBES CISH EBER CISH HPV Low Risk (6 Subtypes) CISH HPV High Risk (18 subtypes) CISH KAPPA CISH LAMBDA Bioz Stars score: 94/100, based on 1 PubMed citations. Technical Overviews English 30 Nov 2021 27.05 MB PDF Return to top Featured References Product Description References 1. VS38C VS38c WT1 6F-H2 CISH EBER CISH HPV Low Risk (6 Subtypes) CISH HPV High Risk (18 subtypes) CISH KAPPA CISH LAMBDA Antibody Clone In addition, VS38c staining of cultures of MG63 and Sa0S2 osteosarcoma cell cultures. VS38C is a mouse anti-human monoclonal antibody that detects a transcription factor produced by normal and malignant plasma cells. doi: 10.1186/s13569-017-0083-5. IMMUNOHISTOCHEMISTRY AND CHROMOGENIC IN SITU (CISH) ACTH polyclonal Actin, Muscle Specific HHF35 Actin Smooth Muscle 1A4 ADH5 DS Breast Cancer Adenovirus M58+M73 . CLIMP-63 is known to act as a spacer that maintains the width of ER sheets. Mouse Anti Vs38c Monoclonal, supplied by Agilent technologies, used in various techniques.
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