Vimentin, also known as fibroblast intermediate filament, is the major intermediate filament found in nonmuscle cells (Colvin et al., 1995 ). Its name comes from the Latin vimentum which refers to an array of flexible rods. cd20 marker pathology outlines. Explore 161 research articles published in the Journal The American Journal of Surgical Pathology in the year 1990. P4 (sarcomatoid carcinoma . Vimentin is a ubiquitously expressed major member of the intermediate filament protein family. Vimentin is expressed in lymphoma tissues. Vimentin expression was . Notably, our multivariate Cox regression model indicated that high Vimentin expression was an adverse prognostic factor for DSS in TSCC patients, even after the adjustment for cell differentiation, pathological stage . Twelve of the 61 B-cell lymphomas were positive for vimentin, and were composed of extrafollicular-center cells such as immunoblastic and plasmacytoid cells. This is applicable to both genders as well as to all age groups from children to adults. Endocervical - ER PR neg, Vimentin Neg, P16 positive, CEA, HPV in situe Positive. Endometrial - ERPR positive, Vimentin positive, P16 negative, HPV in situ negative. Vimentin expression that was equal to or more than 10% of tumor cells with cytoplasmic staining intensity of +1, +2 or +3 was categorized as positive. Aid in diagnosis of tumors of unknown origin and to show nerve sheath involvement Interpretation Cytoplasmic and nuclear staining usually required to call positive Uses by pathologists Marker of schwann cells and melanocytes; useful for evaluating nerve sheath tumors and melanoma Over the lifetime, 8452 publication(s) have been published in the journal receiving 578301 citation(s). Vimentinpositive cases were also stained by . The journal publishes majorly in the area(s): Carcinoma & Adenocarcinoma. A short summary of this paper. The relative areas of immunostaining were measured in three locations of the spinal cord: at the center of the lesion and at sites that were 2 mm rostral . Vimentin plays a role in carcinogenesis through the activation of the epithelial-to-mesenchymal transition (EMT) process. [11] The present case was positive for CD20, vimentin, Bcl2 and Bcl6. These two cases are important for their rarity, especially being vimentin positive, diagnosed on cytology with the aid of immunocytochemistry. We found that vimentin expression was present predominantly in the T cell-rich interfollicular regions of FL (Fig. October 19, 2022. The treatment of tumor cells with a chemotherapeutic agent enhanced the expression of phosphorylated vimentin and augmented the ability of HTL responses against tumor cells. Malignant melanoma. Abstract Alcohol-fixed fine needle aspirates of 82 non-Hodgkin's malignant lymphomas (NHLs) were tested for the presence of vimentin and leukocyte-common antigen (LCA) by means of monoclonal. In recent years, vimentin has been recognized as a marker for epithelial-mesenchymal transition (EMT). Diffuse large B cell lymphoma is one of the most common type of primary thyroid lymphoma. Differentiating lymphoblastic lymphoma and Ewing's sarcoma: lymphocyte markers and gene rearrangement We encountered a child with an intraosseous small round cell tumor that was negative for LCA, CD20 (L26), and CD3 and positive for vimentin, CD99 (MIC-2), and periodic acid-Schiff. Vimentin was occasionally expressed in follicular lymphomas (Fig. . This is in contrast to keratin, which is the intermediate filament found in epithelial cells. Annals of Pathology and Laboratory Medicine. Sagittal sections of the spinal cord of control, vehicle-treated or vimentin-treated SCI mice were immunostained for 5-HT ( a ). AN ULCERO-PROLIFERATIVE LESION OVER LEFT CHEEK-A. Despite the small number of reported cases, patients with positively vimentin-stained NMC tend to follow a similar therapeutic clinical course, in that chemotherapy is initially effective but then fails, followed by a rapid, dismal progression ( 18, 23, 24 ); this is precisely what we observed in the present case. [5] Immunofluorescence staining of HeLa Cells with antibody to reveal vimentin containing intermediate filaments in green and antibody to LAMP1 to reveal lysosomes in red. Sarcomas e.g., fibrosarcoma, malignt fibrous histiocytoma, angiosarcoma, and leio- and rhabdomyosarcoma, as well as lymphomas, malignant melanoma and schwannoma, are virtually always vimentin positive. In diffuse lymphomas, small and medium cell types were more reactive with anti-vimentin than large cell types. However bcl-2 is not useful in distinguishing between types of B cell lymphoma, nor does it help in Conversely, positive N-cadherin and higher Vimentin expression levels were associated with poor DSS and disease-free survival. cytokeratin, 5100 and vimentin were negative. Positive immunohistochemical staining for CD99, CD56, vimentin, NSE and synaptophysin are favorable in the differential diagnosis of PNET ( 6 ). To determine whether vimentin is expressed in lymphoma tissues, we performed immunohistochemical staining of formalin-fixed, paraffin-embedded tissue sections. Vimentin is a 57-kD protein that was initially isolated from a mouse fibroblast culture.2,46 Its name derives from the Latin vimentum, describing an array of flexible rods. The tumor exhibited rosette-like formations. Its prognostic impact in NSCLC has been . Biology of Lymphoma. Two rare cases of primary thyroid lymphoma, both of which are vimentin positive, diagnosed on cytology with the aid of immunocytochemistry, are presented, important for their rarity. . 8). They concluded that a panel consisting of CD99, Terminal deoxy Transferase (TdT) and vimentin along with other lymphocytic markers is helpful in differentiating these two entities.23 However, Terada reported a case of TdT negative, CD99 positive and CD34 positive, T Lymphoblastic lymphoma and suggested inclusion of other precursor cell markers . 2014 Spring;45(2):136-40. doi: 10.1309/lmicz683orlrjrjo. ICC/IF: HeLa, Hap1, SKOV-3 and rat glial cells. This case control study aimed to interpret the expression of vimentin and cytokeratin proteins in the . 6) Woman has recurrent cervix cancer with a picture of a PET showing some . This antibody is a mouse monoclonal IgG1 antibody derived by fusion of PAI Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with vimentin isolated from porcine lens. Get better batch-to-batch reproducibility with a recombinant antibody. All cases of T-cell lymphoma except for 14 (all of 9 AlLD-type lymphomas, all of 4 lymphoblastic lymphomas and one diffuse mixed small/ large lymphoma) were positive for vimentin. Mouse anti-vimentin V9 mAb specific for Vim-C and rabbit anti-vimentin H84 polyclonal Ab specific for Vim-N were used as positive controls. Immunoreactivity with monoclonal antibodies against the intermediate filament protein, vimentin, and epithelial membrane antigen (EMA) was examined in 330 cases of lymphoma (317 nonHodgkin's and 1. Bcell Lymphoma with Vimentinpositive Cytoplasmic Inclusions - Lertprasertsuke - 1991 - Pathology International - Wiley Online Library 2), and the proportion of positive cases (11.5%)'was markedly lower than in diffuse B-cell lymphomas, 25.6% of which expressed vimentin. Cervical cancer is one of the commonest types of cancers worldwide especially in developing countries. Vimentin is a 57 kDa intermediate filament protein which forms part of the cytoskeleton of vertebrate cells and is characteristically found in cells of mesenchymal origin. October 19, 2022 It marks most soft tissue lesions and malignant melanoma. Intermediate filaments protein family has shown a role in the diagnosis of various cancers, but a few studies are available about the vimentin and cytokeratin roles in the cervical cancer. The coexpression of intermediate filaments, particularly vimentin and cytokeratin, has been demonstrated in a variety of normal cells . Two young adult Macaca fascicularis each had unilateral mydriasis and ptosis. Successive studies on human lymphomas have shown that lymphoma cells also retain vimentin, although the extent of immunoreactivity is variable (5-8 . Despite the abundance of clinical data linking vimentin to poor patient outcome, it is unclear if vimentin is required for metastasis or is a correlative biomarker. Vimentin IF proteins have been im. The lymphoma was classified as follicular type, of B celllineage with signet-ring features. Vimentin expression in follicular lymphomas was less frequent than in diffuse B-cell lymphomas. All LCALs were CD30+, and, where tested, vimentin positive. Statistical analysis. Purchase matching WB positive control: Recombinant Human Vimentin protein; WB: A549, Hap1, HeLa, U-2 OS, human tonsil and MOLT4 whole cell lysates. Vimentin, also known as fibroblast intermediate filament, is the major intermediate filament found in nonmuscle cells (Colvin et al., 1995 ). Vimentin (Autostainer Link 48) Clone V9. Recently, the expression of vimentin has been reported in some carcinomas. B-cell lymphoma with vimentin-positive cytoplasmic inclusions. Of four different vimentin monoclonal antibodies tested, V9 and MVI stained the highest number of lymphomas. Diagnosis of mantle cell lymphoma: up to 98% positive (Am J Hematol 2018;93:E134) Differentiating clear cell sarcoma of kidney (positive for cyclin D1) from blastema rich Wilms tumor (blastemal component is negative for cyclin D1) (Diagn Pathol 2019;14:13) Differentiating adenocarcinoma from small cell carcinoma of prostate (shows cyclin D1 loss) (Clin Cancer Res 2015;21:5619) Although vimentin expression appeared to be influenced by various conditions such as the proportion of T- and 6-cell subsets, or B-cell proliferation rate, follicular . Long-term and scalable supply - powered by recombinant technology for fast production. This difference in reactivity was statistically significant (P<0.05). It was first reported in 1997 by Delsol [ 1 ], and no more than 100 cases have been reported up to now. BackgroundCurrent therapy for non-small-cell lung cancer (NSCLC) frequently includes immune checkpoint inhibitors, such as pembrolizumab, and programmed death ligand 1 (PD-L1) positivity is mandatory for its use in this setting. Reed-Sternberg cells (R-S cells) in Hodgkin's disease were positive for vimentin in 11 cases (84.6%). Statistical analysis Although vimentin expression appeared to be influenced by various conditions such as the proportion of T and B cell subsets, or B cell proliferation rate . This antibody reacts exclusively with vimentin, which is expressed in mesenchymal cells and mesenchymal derived tumors e.g. Lymphomas can be positive for vimentin but are negative for keratin and desmin, which are characteristic markers for benign and malignant epithelial or muscular tissues respectively Vimentin-negative cases included Burkitt's as well as some follicular lymphomas. Primary body cavity-based large B-cell lymphoma in an HIV and HHV-8 negative, HCV positive patient: a case report and literature review Lab Med . This is in contrast to keratin, which is the intermediate filament found in epithelial cells. The SMA-, vimentin- and desmin-positive cells were counted from six different corneas in the six randomly selected, non-over-lapping, full-thickness central corneal columns under 400 microscopic field, as previously described (Mohan et al., 2003). Purpose: Vimentin is an epithelial-to-mesenchymal transition (EMT) biomarker and intermediate filament protein that functions during cell migration to maintain structure and motility. Vimentin is present in many different neoplasms but is particulary expressed in those originated from mesenchymal cells. There is an accumulation of evidence in the literature demonstrating the integral role of vimentin intermediate filaments (IFs) in the progression of lung cancers. This IFP is considered to be the primordial member of the intermediate filament family, because it is present in most, if not all, fetal cells early in development. Vimentin's overexpression in cancer correlates well with accelerated tumor growth, invasion, and poor prognosis; however, the role of vimentin in cancer progression remains obscure. Paranuclear blue inclusions in metastatic rhabdomyosarcoma of the bone marrow A 13-year-old boy with a history of left knee joint and back pain presented with severe discomfort, inguinal. This Paper. Vimentin enhances the 5-HT-positive axonal density in SCI mice. The cleaved centrocytic lymphoma cells were immunoreactive for . All slides were evaluated by a pathologist (K.R) who was blinded from patient outcomes. follicular lymphoma are bcl-2 positive. cd20 marker pathology outlines. Vimentin staining confirms mesenchymal origin of some tumors but there are numerous exceptions, so relatively nonspecific Carcinomas and epithelial tumors are usually negative but there are many exceptions Thus, helps distinguish renal cell carcinoma and uterine carcinoma as part of panel Pathophysiology Neuroblastomas are also positive for NSE and synaptophysin, but negative for CD99, and the presence of Homer-Wright rosettes is a characteristic of these lesions ( 14 ). The biopsy diagnosis of the cervical lymph nodes was B-cell lymphoma, diffuse medium-sized cell type. . Positive staining of tumor cells was seen in 61 of 71 cases. The lymphoid origin of these latter cases was proven by gene rearrangement studies. Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547) Research with confidence - consistent and reproducible results with every batch. Contents 1 Positive 1.1 Vimentin and cytokeratin positivity 2 Negative 3 See also 4 References Positive Most sarcomas. Lymphoma is a collection of cancers arising from the malignant transformation of lymphocytes. It is strongly expressed in a broad range of mesenchymal cells, and in more limited subsets of normal epithelial cells. lymphoma, sarcoma and melanoma. Vimentin is a common immunostain. It was first recognized by Stein et al 1 in 1985, who described 45 cases with CD30 (Ki-1 antigen) expression and prominent sinusoidal invasion that had previously been diagnosed as malignant histiocytosis or anaplastic carcinoma. vimentin positive Differential Treatment Nonoperative chemotherapy alone indications only indicated in select patients with widespread metastatic disease. Antibodies to intermediate filament proteins (vimentin, keratin, and desmin) are also useful to distinguish between lymphoma and other neoplasms. 2.5. This study was designed to clarify the significance of vimentin expression in solid type poorly differentiated adenocarcinomas of the stomach. 1 for progressive neurologic signs and monkey No. Primary thyroid lymphomas are rare tumours of thyroid accounting for 5% of all thyroid tumours. In the present study, we identified phospho-peptide epitopes from vimentin that could elicit an effective HTL response. Malignant lymphoma involving systemic lymph nodes and the bilateral tonsils was suspected by computed tomography. 5) List 4 immuno markers to help distinguish between endometrial and endocervical adenocarcinoma. ALCL is a type of non-Hodgkin T-cell lymphoma that can involve lymph nodes and uniformly expresses CD30. Vimentin Positive Primary Thyroid Diffuse Large B-Cell Lymphoma: cytological diagnosis of two cases. All LCALs were CD30+, and, where tested, vimentin positive. The frequency of EMA reactivity in lymphomas was low, particularly in T-cell lymphomas. 2 because of a positive in. It is strongly expressed in a broad range of mesenchymal cells, and in more limited subsets of. Over the lifetime, 8845 publication(s) have been published in the journal receiving 277816 citation(s). Anti-epidermal growth factor receptor Ab, cetuximab . IHC-P: Human kidney, Hodgkin's Lymphoma and oral cavity tissue sections. Keywords: Thyroid, Cytology, FNAC, Lymphoma, Diffuse . Full text [1] Lymphomas - many. All cases of T cell lymphoma except for 14 (all of 9 AlLD type lymphomas, all of 4 lymphoblastic lymphomas and one diffuse mixed small/ large lymphoma) were positive for vimentin. The journal publishes majorly in the area(s): Carcinoma & Adenocarcinoma. Vimentin positivity is also considered as a poor prognostic factor. Even though lymphoma is clinically a diverse group of neoplasms, the common origin is the lymphoreticular cells. The normal result for this test is if the results show a positive detection of the Mesenchymal Origin Tumor. It is usually negative in carcinomas. The coexpression of intermediate filaments, particularly vimentin and cytokeratin, has been demonstrated in a variety of normal cells/tissues and in neoplastic lesions, necessitating the use of antibodies against other types . Intratumoral heterogeneity of cytokeratin and vimentin expression for two representative anaplastic lymphoma kinase (ALK) -positive patients bearing either a prominent mesenchymal phenotype (P4) or a prominent epithelial phenotype (P17) and for whom ALK expression protein could be assessed by immunohistochemistry. Flow Cyt: HAP1, MDA-MB-231 and SV40LT-SMC cells. Download Download PDF. These cell types include fibroblast, endothelial cells, macrophages, melanocytes, Schwann cells, and lymphocytes. These cell types include fibroblast, endothelial cells, macrophages, melanocytes, Schwann cells, and lymphocytes. Explore 125 research articles published in the Journal Histopathology in the year 1987. Charanjeet Ahluwalia. [2] [3] SHARE. The vimentin immunohistochemistry test is used to detect tumors or carcinomas, and hence is a control marker used in the diagnosis of cancer. recently, an in vitro model of keratin and vimentin coexpression in breast cancer cells (developed as stable transfectants) has revealed that these keratin/vimentin if-positive cells display increased proliferation rates, invasive potential, clonogenicity, and tumorigenicity when compared with keratin-positive/vimentin-negative controls Full PDF Package Download Full PDF Package. Vimentin is a 57 kDa intermediate filament protein which forms part of the cytoskeleton of vertebrate cells and is characteristically found in cells of mesenchymal origin. Anaplastic lymphoma kinase (ALK)-positive diffuse large B-cell lymphoma (DLBCL) is a rare subtype of non-Hodgkin's B-cell lymphoma, which is characterized by plasmablastic or immunoblastic morphology. Positive staining of tumor cells was seen in 61 of 71 cases. Reed-Sternberg cells (R-S cells) in Hodgkin's disease were positive for vimentin i n 11 cases (84.6%). Success from the first experiment . Vimentin is a structural protein that in humans is encoded by the VIM gene. Of four different vimentin monoclonal antibodies tested, V9 and MVI stained the highest number of lymphomas. radiation therapy alone indications can be used for unresectable tumors or close/positive margins Operative wide surgical excision with chemotherapy indications pediatric rhabdomyosarcoma On the other hand, lymphomas of follicular center cell origin were negative for vimentin. Lymphoma is one of the most common canine cancers, accounting for 7-24% of all canine tumors and 85% of . Immunohistochemically, 239 poorly differentiated adenocarcinomas with solid components of the stomach were stained for vimentin. Briefly, the staining intensity was determined by cytosolic staining for vimentin. Malignant lymphoma involving systemic lymph nodes and the bilateral tonsils was suspec. Both animals were euthanatized, monkey No. . Vimentin staining in a lymphoma. Vimentin is a ubiquitously expressed major member of the intermediate filament protein family.
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