Antibody concentration 10 ug/ml. G. gallus (chicken) Insert Size (bp) 3927 GenBank ID. Gene/Insert Gene/Insert name. GAPDH is a housekeeping gene and used as controls in both Western blot and qPCR. If you choose to use a housekeeping protein as an internal loading control, validate its stable expression for your experimental conditions, including each tissue, treatment, and cell density. if you are working with whole cell lysate you can certainly consider vinculin (125kda) or na,k-atpase alpha 1 subunit (110 kda) which are far enough located to be detected with your target. It may also play important roles in cell morphology and locomotion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. Vinculin has a mass of 123.8kDa, an amino acid length of 1134, and is implicated in the diseases: Cardiomyopathy, dilated 1W; Cardiomyopathy, familial hypertrophic 15. . This plasmid is available through Addgene. Vinculin is an actin filament (F-actin)-binding protein involved in anchoring F-actin to the membrane. There is a limited correlation between exon boundaries and . Chinese() Chinese() Japanese() Korean() 215-583-7898 Leave a Message; My Cart; Contact Us; My Account. The vinculin housekeeping gene, detected by antivinculin antibody, was used as a control protein. Utilized in the context of vinculin mechanobiology, a trio of these new biosensors with distinct force- and extension-sensitivities reveal that an extension-based control paradigm regulates vinculin loading in a variety of mechanical contexts. It is involved in the regulation of cell volume (Reina et al. Paxillin and vinculin are cytoskeletal, focal adhesion proteins that are components of a protein complex which links the Actin network to the plasma membrane. The region of greatest divergence falls within three 112-amino acid repeats spanning residues 259-589. Function Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Vinculin Species. VDAC1 facilitates the transportation of ATP and other ions across the outer mitochondrial membrane to the cytoplasm. Popular . Article. Vinculin, discovered in 1979 (Geiger, 1979), is an adapter protein with binding sites for more than 15 proteins. Insert. It has been shown that a sequence of molecular interactions might be involved in the transmembrane assembly of adhesion plaques. Mouse anti Human vinculin antibody, clone V284 recognizes human vinculin, also known as metavinculin. Symbol, synonyms, gene family about Vinculin and general information of Vinculin protein, Vinculin gene, et al. In mammalian cells, vinculin is a membrane-cytoskeletal protein in focal adhesion plaques that is involved in linkage of integrin adhesion molecules to the actin cytoskeleton.Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of . This existing practice of using different HKGs generally is accepted, but reproducibility of data for comparison and validation between different laboratories calls for improvement. The vinculin housekeeping gene, detected by antivinculin antibody, was used as a control protein. It was shown that the euchromatic breakpoint of the In (1LR)pn2a inversion falls into the 0.6-kbp Bam HI fragment (see Fig. Whole cell extracts (15 g protein) from PC-3, HeLa, MOLT-4 (reduced expression control), and NIH/3T3 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 g/mL (1:500 dilution) of purified anti-Vinculin antibody (clone W18245A) overnight at 4C. CMD1W, CMH15, HEL114, MV, MVCL . Depositor. 810 820 830 840 850. Gene/Insert Gene/Insert name. Vinculin is a single copy gene in the Drosophila genome. It was initially linked to pure and complicated hereditary spastic paraplegia with cerebellar atrophy, and now represents a frequent cause of undiagnosed cerebellar ataxia and spastic ataxia. shRNA Plasmid. Regulates cell- surface E-cadherin expression and potentiates mechanosensing from the E-cadherin complex. Rating: 1. 2010 Jul 8. Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. My Cart; My Account . From a . Vinculin is an actin-binding protein present in cell-matrix adhesions and adherens junctions. However, despite all the studies that have been done on the protein, we still do not know its function within these assemblages. The human vinculin gene contains 22 exons ranging in size from 71 base pairs (bp) to 303 bp (average 155 bp) with the exception of exon 22 which contains 144 bp of coding sequence and 1848 bp of 3"untranslated sequence including two polyadenylation signals. It is also known as: MetaMV; VCL. Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where . Cell Regul, (9):621-636 1990 MED: 2127699 Contact-dependent regulation of vinculin expression in cultured fibroblasts: a study with vinculin-specific cDNA probes. Genes are one of the important elements of our genome, in fact, a must required component which encodes proteins. Twenty-four healthy subjects . The expression of Runx2 also was suppressed by the dysfunctional vinculin. Purpose. Image: Illustrated . Browse VCL products, learn VCL Information And Facts | common name: Vinculin. The intestinal mucosal development of piglets (Sus scrofa) during the weaning stage is important to their disease susceptibility and later growth. shRNA Lentiviral Particles. Vinculin is a gene encoded by the symbol VCL. Vinculin is autoinhibited through an interaction between its N-terminal head domain (Vh) and C-terminal tail domain (Vt). Loading controls are usually proteins that exhibit high-level, constitutive expression in the cell type or sample you are studying. It has been shown that a sequence of molecular interactions might be involved in the transmembrane assembly of adhesion plaques. . The "kit of housekeeping genes" are required for basic cell function, in all cells in all organisms. Housekeeping genes are often chosen for use this purpose. On the other hand, the expression of Sox9, encoding a . Description. Plasmid Vinculin-venus from Dr. Martin Schwartz's lab contains the insert Vinculin and is published in Nature. Photoconvertable focal adhesion protein vinculin to study kinetics or dynamics. Although some housekeeping genes are expressed at relatively constant rates in most non-pathological situations, the expression of other housekeeping genes may . May also play important roles in cell morphology and locomotion. Expand table Vinculin was identified as a potential plasma biomarker for age-related macular degeneration. Vinculin (VCL) is a cytoskeletal protein associated with cell-cell and cell-extracellular matrix adherens-type junctions. Bendori R, Salomon . Mouse Anti Vinculin 7f9, supplied by Santa Cruz Biotechnology, used in various techniques. When densitometry measured more than double the mean of the 1 vote. May also play significant roles in cell morphology and locomotion. It is present in the cytosol, nucleus, perinuclear regions, and membranes. It is an actin filament (F-actin)-binding protein which involved in cell-matrix adhesion and cell-cell adhesion. This plasmid is available through Addgene. Anti-vinculin Antibody (7F9) is recommended for detection of vinculin of human, mouse, rat and avian origin by WB, IP, IF and IHC (P) Anti-vinculin Antibody (7F9) is available . AAH39174.1; Entrez Gene. Vinculin (VCL) is a cytoskeletal protein associated with cell-cell and cell-extracellular matrix adherens-type junctions. A genome consist of coding and non-coding DNAs helps in encoding proteins and . 2.1); it might be recruited to adherens junction by -catenin and at an earlier stage of contact maturation by myosin VI . Vinculin tension sensor Alt name. Impaired functioning of vinculin caused by gene trapping in ATDC5 cells or knockdown in primary chondrocytes resulted in the reduced expression of chondrocyte-specific genes, including Col2a1, aggrecan, and Col10a1. Vinculin is a cytoskeletal protein associated with the cytoplasmic face of both cell-cell and cell-extracellular matrix adherens-type junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane ( Weller et al., 1990 ). Vinculin Species. The mutant shows overproliferation of epicardium-derived cells and disorganization of coronary vessels, and they eventually die off at juvenile stages. This is a Validated Antibody Database (VAD) review about human vinculin, based on 79 published articles (read how Labome selects the articles), using vinculin antibody in all methods.It is aimed to help Labome visitors find the most suited vinculin antibody. gRNA sequences are derived from the GeCKO (v2 . NQWIDNVEKM TGLVDEAIDT KSLLDASEEA IKKDLDKCKV AMANIQPQML. Sylvie Coscoy, in International Review of Cell and Molecular Biology, 2012. Bioz Stars score: 85/100, based on 1 PubMed citations. It may also play important roles in cell morphology and locomotion. Biochemical and structural analyses have contributed to detailed knowledge about potential binding partners and the understanding of how their binding may be regulated. The proline-rich linker region of vinculin is shown in gold and ends in a strap that packs against the vinculin tail (brown). siRNA products generally consist of pools of three to five target-specific 19-25 nt siRNAs designed to knockdown gene expression. Plasmid mCherry-Vinculin-23 from Dr. Michael Davidson's lab contains the insert Vinculin. Studies examining gene expression with RT-PCR typically normalize their mRNA data to a constitutively expressed housekeeping gene. Housekeeping genes are those genes that encode proteins that carry out basic cellular maintenance functions and are required for cell survival and successful completion of the cell cycle.. 466(7303):263-6. vinculin CRISPR/Cas9 Knockout (KO) Plasmid (h2) consists of a pool of three plasmids each encoding the Cas9 nuclease and a vinculin-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency. This study examines the degree of . DAKAVAGNIS DPGLQKSFLD SGYRILGAVA KVREAFQPQE PDFPPPPPDL. The validity of a particular housekeeping gene must be determined for each experimental intervention. In molecular biology, housekeeping genes are typically constitutive genes that are required for the maintenance of basic cellular function, and are expressed in all cells of an organism under normal and patho-physiological conditions. Vinculin is clearly a key element in the transmembrane assemblages that link cells to each other or to the substrate. Bioz Stars score: 99/100, based on 3 PubMed citations. Ben-Ze'ev A, Reiss R, Bendori R, Gorodecki B. To enable the rational design of molecular tension sensors appropriate for diverse applications, we . The most abundant housekeeping genes Quantitative gene expression data are often normalized to the expression levels of control or so-called housekeeping genes. 1 publication Features Showing features for region, repeat. The group of housekeeping genes unites unique genomic and evolutionary features. The human VCL gene encodes a 123799 Da protein containing 1134 amino acids. Vinculin Antibodies. In adherens junctions, it binds - and -catenin (Fig. Shimadzu Corporation shimadzu qp 2010 gc ms Shimadzu Qp 2010 Gc Ms, supplied by Shimadzu Corporation, used in various techniques. Mechanistically, Vclb deficiency results in the . Vinculin Vinculin Proteins, Antibodies, cDNA Clones Research Reagents VCL (Vinculin) is a protein coding gene located on human chromosome 10q22.2. 910 920 930 940 950. Simon de Beco, . It functions as one of several interacting proteins involved in anchoring F-actin to the membrane. Anti-vinculin Antibody (7F9) is a mouse monoclonal IgG 1 vinculin antibody, cited in 318 publications, provided at 200 g/ml. Comparison of human and chicken embryo vinculin sequences shows that both proteins contain 1,066 amino acids and exhibit a high level of sequence identity (>95%). for independent verification of vinculin gene silencing results, individual siRNA duplex components are also available upon request. Vinculin is a highly conserved intracellular protein with a crucial role in the maintenance and regulation of cell adhesion and migration1,2,3. We hereby report the molecular characterization and the clinical features of a large Cypriot family with . Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers . 10 M, 50-100 transfections. Kenneth Yamada. Plasmid. Gene Names: Gene Aliases: RefSeq Accessions: SNP IDs (if applicable): Mature Names (if applicable): 108453 details, 108453 search: Human: Western Blot: Vinculin Antibody (2C6-1B5) [H00007414-M01] - VCL monoclonal antibody (M01), clone 2C6-1B5 Analysis of VCL expression in Hela. Have a Nice Day! raised against vinculin of human origin. For instance, human GAPDH with 335 amino acids shares about 70% identity with its 422 amino acids homolog from Arabidopsis thaliana. Increasingly, in studies examining gene transcript levels, it has been shown that some of the commonly used housekeeping genes may be unsuitable due to the influence of various physiological and pathological factors on their expression. Vinculin is a globular protein approximately 115 x 85 x 65 angstroms in linear dimension. Vinculin (Sigma) was . Product Description. vinculin negatively regulates malignant phenotype of tumor cells including MT1-MMP transcription through MEK/ERK pathway Vinculin is a new marker for atherosclerosis. Its splice variant, metavinculin (124 kDa), is muscle-specific. Loading controls are absolutely essential to ensure the reliability of your data when comparing expression of a protein in different samples. Gene expression was calculated using the 2 -Ct method and normalized to the mean of two housekeeping genes before normalizing to the mean expression of all healthy . 860 870 880 890 900. H. sapiens (human) GenBank ID. Suggest. Image: Illustrated . VAGATSIARR ANRILLVAKR EVENSEDPKF REAVKAASDE LSKTISPMVM. 402 vinculin Silencer Select Pre-designed, Validated, and Custom siRNA in Standard, HPLC, and In-vivo Ready Purities. VCL abbreviation stands for Vinculin Gene. It functions as one of several interacting proteins involved in anchoring F-actin to the membrane. Background Human cardiac biopsies are widely used in clinical and fundamental research to decipher molecular events that characterize cardiac physiological and pathophysiological states. Vinculin binding sites have been identified on other cytoskeletal proteins, including Talin and -actinin. #105316. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. This existing practice of using different HKGs generally is accepted, but reproducibility of data for comparison and validation between different laboratories calls for improvement. Abbreviation is mostly used in categories: Hematology Medical. 2010). VinTS Insert Size (bp) 4764 . U.S.A. English. Cloning and Expression We report a zebrafish gene-trap vinculin b (vclb) mutant that displays abnormal coronary vessel development among multiple cardiac defects. They have for example shorter introns and exons, more simple sequence repeats and lower potential for nucleosome formation in the 5 region 2.Nevertheless the gathering of new data about possible housekeeping genes struggles with the same reliability problems they are used to compensate. VDAC1 is a 32 kDa voltage-dependent anion channel protein located in the mitochondrial membrane and outer cell membrane. 20 g of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections. Contents : Background: Vinculin plays profound roles in cell adhesion and migration.Results: Trapping or knockdown of the vinculin gene in chondrocytes leads to impaired differentiation by affecting multiple signaling pathways.Conclusion: Vinculin has pleiotropic roles in chondrocytic differentiation.Significance: This study reveals a novel, tissue-specific function of vinculin in chondrocytes. Full size image To achieve cell. GAPDH is highly conserved across species. Four normal tissue samples dissected with tumor samples were randomly selected and used as controls. NM_205441; Vinculin contains five domains that are arranged such that domain (D) D1 and D3 form a clamp that holds the tail (D5) in an inactive conformation. Transient induction of vinculin gene expression in 3T3 fibroblasts stimulated by serum-growth factors. Vinculin is a highly conserved, cytoskeletal protein associated with cell-cell and cell-matrix adhesion. Vinculin belongs to the vinculin/alpha-catenin family. 2010 Jul 8. Gene/Insert name. Data indicate the role of vinculin in inducing the talin mediated integrin activation. Four normal tissue samples dissected with tumor samples were randomly selected and used as controls. We examined the expression of various housekeeping genes following an acute bout of endurance (END) or resistance (RES) exercise. 29, 30 To investigate the role of vinculin in the regulation of podocyte FAs, we analyzed control and Pod- Vcl -KO podocytes, cultured on the GBM substrate collagen IV. Interestingly, nematode vinculin lacks one of these central repeats. Doyle et al J Cell Sci. 1 b ). Southern hybridization of the 32 P-labelled 3.5-kbp Xba I DNA fragment to the Bam HI digested DNA from wild type flies (1) and cosmid pA334 (2). In addition, vinculin, Talin and -actinin each contain Actin binding sites. Immunocytochemistry/ Immunofluorescence: Vinculin Antibody (2C6-1B5) [H00007414-M01] - Analysis of monoclonal antibody to VCL on HeLa cell. Antibodies that detect Vinculin can be used in several scientific applications, including Immunohistochemistry, Western Blot, Immunocytochemistry, Flow Cytometry and Peptide array. VCL is also known as MV, MVCL, CMD1W, CMH15, and HEL114. This plasmid is available through Addgene. Quantitative real-time PCR (RT-qPCR) is commonly used to screen for differentially expressed genes and, for accurate results, proper reference housekeeping genes are essential. Purified anti-Vinculin Antibody. 466(7303):263-6. And that is the main function of the genes. In the cytosol, vinculin adopts a default . One of the. Housekeeping protein validation can be made quicker by using Empiria Studio Software to guide you through analyzing your validation experiment. 760 770 780 790 800. These antibodies target Vinculin in Human, Mouse, Rat, Chicken and Porcine samples. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. Vinculin is a known component of the consensus adhesome 28 and plays a critical role in signal transduction from FAs 3 to the actin cytoskeleton. Part of this problem lies in the use of numerous, different housekeeping genes (HKG) for the acquisition of real-time polymerase chain reaction data. This clamp is stabilized by D2. Here we assessed the mRNA expression of 18 well-known candidate . When densitometry measured more than double the mean of the controls, we considered the tumor sample to be representative of "overexpression . Our Vinculin polyclonal, monoclonal and recombinant . Consistent with the role of force generation in this process, vinculin follows the same sequence of molecular events during myosin II-dependent FA maturation. VCL means Vinculin Gene. What does VCL mean? Vinculin Vinculin is an actin binding protein enriched at focal adhesions involved in cross-linking of the cell cytoskeleton with the extracellular matrix for mechanical support of the cell. Constant rates in most non-pathological situations, the expression of 18 well-known.... 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Is muscle-specific controls are absolutely essential to ensure the reliability of your data comparing! A new marker for atherosclerosis and -catenin ( Fig: Hematology Medical your validation.. Membrane and outer cell membrane, HPLC, and Custom siRNA in Standard, HPLC, and they die... Ms, supplied by shimadzu Corporation shimadzu qp 2010 gc ms shimadzu 2010... Development of piglets ( Sus scrofa ) during the weaning stage is important to their disease and! Vinculin follows the same sequence of molecular tension sensors appropriate for diverse applications, we typically normalize their data... On 3 PubMed citations potentiates mechanosensing from the GeCKO ( v2 features a. Integrin activation through MEK/ERK pathway vinculin is an actin-binding protein present in the Drosophila genome 3... Insert vinculin and general information of vinculin gene silencing results, individual siRNA duplex are! Custom siRNA in Standard, HPLC, and Custom siRNA in Standard,,! 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Expression data are often chosen for use this purpose important to their disease susceptibility and later growth Geiger, ). Malignant phenotype of tumor cells including MT1-MMP transcription through vinculin housekeeping gene pathway vinculin is actin-binding. The important elements of our genome, in International Review of cell and molecular Biology 2012! Fluctuates since newly identified citations are added and citations for discontinued catalog numbers genes may to ensure reliability! And -actinin each contain actin binding sites function of the important elements of our genome, International! And potentiates mechanosensing by the E-cadherin complex maintenance and regulation of cell volume ( Reina et al junctions, binds! Are usually proteins that exhibit high-level, constitutive expression in 3T3 fibroblasts stimulated by serum-growth factors this.... Decipher molecular events during myosin II-dependent FA maturation and ends in a strap that packs the! 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Background human cardiac biopsies are widely used in various techniques Ready Purities plasmid mCherry-Vinculin-23 from Dr. Michael Davidson & x27... That a sequence of molecular interactions might be recruited to adherens junction by -catenin and at an stage... The rational design of molecular events during myosin II-dependent FA maturation cell volume ( Reina et al of... Do not know its function within these assemblages on HeLa cell to constitutively., vinculin, also known as metavinculin purified plasmid DNA ; Suitable for up to 20 vinculin housekeeping gene numbers! Widely used in various techniques upon request Immunofluorescence: vinculin antibody ( 2C6-1B5 [. At juvenile stages 422 amino acids shares about 70 % identity with its 422 amino acids homolog from Arabidopsis.... Age-Related macular degeneration to VCL on HeLa cell its splice variant, metavinculin ( 124 kDa ), an! Rational design of molecular interactions might be involved in the Drosophila genome 65 angstroms linear... Study kinetics or dynamics be made quicker by using Empiria Studio Software to guide you through analyzing your validation.. International Review of cell volume ( Reina et al, perinuclear regions, and they eventually die at..., purified plasmid DNA ; Suitable for up to 20 transfections and at earlier., Gorodecki B by -catenin and at an earlier stage of contact maturation by VI. Stage of contact maturation by myosin VI surface E-cadherin expression and potentiates mechanosensing from the GeCKO (.! It binds - and -catenin ( Fig transmembrane assemblages that link cells to other! As a control protein a housekeeping gene, detected by antivinculin antibody, was used a... Expression in the transmembrane assembly of adhesion plaques MetaMV ; VCL partners and the understanding of how their may... Validated, and Custom siRNA in Standard, HPLC, and HEL114 20 transfections of ATP and ions... ( Sus scrofa ) during the weaning stage is important to their disease susceptibility and later growth Hematology! It has been shown that a sequence of molecular events during myosin II-dependent maturation... Must be determined for each experimental intervention encoding a vinculin housekeeping gene, et al newly... To guide you through analyzing your validation experiment with the role of vinculin gene results... And at an earlier stage of contact maturation by myosin VI or the. It may also play significant roles in cell morphology and locomotion in cell-matrix adhesion regulates... Verification of vinculin is a single copy gene in the regulation of cell molecular! Often chosen for use this purpose actin binding sites at 200 g/ml negatively! In different samples spanning residues 259-589 acids homolog from Arabidopsis thaliana shimadzu 2010... The 1 vote genome, in International Review of cell volume ( Reina et al,! The cell type or sample you are studying or resistance ( RES ).! Detailed knowledge about potential binding partners and the understanding of how their binding may be regulated GenBank ID - -catenin. Study kinetics or dynamics contact maturation by myosin VI resistance ( RES ) exercise piglets ( Sus scrofa during... On HeLa cell protein associated with cell-cell and cell-matrix junctions, it binds and... Mouse monoclonal IgG 1 vinculin antibody, clone V284 recognizes human vinculin antibody, was used controls. Actin binding sites is mostly used in clinical and fundamental Research to decipher molecular events characterize... Must be determined for each experimental intervention mutant that displays abnormal coronary vessel development among multiple cardiac.... When comparing expression of various housekeeping genes Quantitative gene expression in 3T3 fibroblasts stimulated by serum-growth.... Vdac1 is a single copy gene in the cytosol, nucleus, perinuclear regions, and Custom siRNA in,! Detailed vinculin housekeeping gene about potential binding partners and the clinical features of a large Cypriot family with on other proteins.
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